The role of CD38 in Fcγ receptor (FcγR)-mediated phagocytosis in murine macrophages. > 연구자료실

본문 바로가기

연구자료실

연구자료실
IP: ***.***.***.4.1

The role of CD38 in Fcγ receptor (FcγR)-mediated phagocytosis in murin…

페이지 정보

최고관리자 조회수 : 1,032

본문

J Biol Chem. 2012 Apr 27;287(18):14502-14. doi: 10.1074/jbc.M111.329003. Epub 2012 Mar 6.

The role of CD38 in Fcγ receptor (FcγR)-mediated phagocytosis in murine macrophages.

Abstract

Phagocytosis is a crucial event in the immune system that allows cells to engulf and eliminate pathogens. This is mediated through the action of immunoglobulin (IgG)-opsonized microbes acting on Fcγ receptors (FcγR) on macrophages, which results in sustained levels of intracellular Ca(2+) through the mobilization of Ca(2+) second messengers. It is known that the ADP-ribosyl cyclase is responsible for the rise in Ca(2+) levels after FcγR activation. However, it is unclear whether and how CD38 is involved in FcγR-mediated phagocytosis. Here we show that CD38 is recruited to the forming phagosomes during phagocytosis of IgG-opsonized particles and produces cyclic-ADP-ribose, which acts on ER Ca(2+) stores, thus allowing an increase in FcγR activation-mediated phagocytosis. Ca(2+) data show that pretreatment of J774A.1 macrophages with 8-bromo-cADPR, ryanodine, blebbistatin, and various store-operated Ca(2+) inhibitors prevented the long-lasting Ca(2+) signal, which significantly reduced the number of ingested opsonized particles. Ex vivo data with macrophages extracted from CD38(-/-) mice also shows a reduced Ca(2+) signaling and phagocytic index. Furthermore, a significantly reduced phagocytic index of Mycobacterium bovis BCG was shown in macrophages from CD38(-/-) mice in vivo. This study suggests a crucial role of CD38 in FcγR-mediated phagocytosis through its recruitment to the phagosome and mobilization of cADPR-induced intracellular Ca(2+) and store-operated extracellular Ca(2+) influx.

대표 : 김좌진 ㅣ 개인정보관리 : 김좌진 ㅣ 사업자번호 : 253-86-01081 ㅣ 통신판매업신고: 2018-대전동구-0190호
본사 : 대전광역시 동구 충정로 21, 4105호 ㅣ 기업부설연구소 : 대전광역시 동구 충정로 21, 4104호
당사는 모든 이미지의 무단사용을 금하며, 무단사용시 저작권법 98조에 의거 민형사상 책임을 지게됩니다.
COPYRIGHT ©2019 (주)프로셀컴퍼니. ALL RIGHTS RESERVED.